The State Institution
Romodanov Neurosurgery Institute
National Academy of Medical Sciences of Ukraine

Neuroimmunology Department

Mykola I. Lisianiy +380 44 483-81-93


Staff  |  Publications  |  Diagnostic methods  |  Types of pathology  |  Counseling  |  Contacts

About the department

The neuroimmunology department was set up in 1985 as part of an immunology laboratory of the Institute of Neurosurgery. The department is a leading scientific subunit in the field of neuroimmunology of Ukraine, a center that develops and implements modern immunological and genetic methods of laboratory diagnosis in patients with CNS pathology. The department has developed and introduced immunoferment methods to assess formations of antibodies to cerebral tissue proteins, as well as classical immunological methods to assess the immune status and immune humoral response. Methods of molecular-biological research, analysis of gene expression in peripheral blood cells and CNS tumors are actively introduced. The department staff take part in international scientific forums and establish contacts with counterparts from other institutions.

Head of the department – honored science and technology worker of Ukraine, corresponding member of the National Academy of Medical Science of Ukraine, PhD, Professor Mykola Ivanovych Lisianiy.

О.V.Markova and I.O.Hnedkova, candates of medical science, and L.D.Liubych and L.M.Belska, candidates of biological sciences, as well as A.I.Kliuchkova, a research assistant, work in the department.

Staff

Professor Lisianiy Mykola Ivanovych – an honored science and technology worker of Ukraine, corresponding member of the National Academy of Medical Science of Ukraine, PhD head of the neuroimmunology department. Key areas of research and practice include neuroimmunology, neurogenetic immunodeficiency, immunopathological mechanisms of development of neuropathies, neuroimmune mechanisms of radiation-induced encephalopathy development under the effect of small doses of irradiation. Under his guidance 3 doctoral and 19 candidate dissertations were completed. Author of 400 scientific works, 9 monographs.

Senior researcher Markova Olha Volodymyrivna –candidate of medical science. Key areas of research and practical activity include the immune status of a healthy human being; anti-tumour immunity; pathophysiology inflammatory-degenerative damage of CNS and mechanisms of therapeutic effect of neurotransplantation; development of methods to use hemopoietic stem cells in neurology and neurosurgery. Author of 109 scientific works, including 19 publications abroad, 6 patents of Ukraine, 3 technical innovations, co-author of 4 monographs.

Senior researcher Gnedkova Iryna Oleksandrivna – candidate of medical science, immunologist of the highest qualification category in the specialty “clinical immunology”. Key areas pf research and practical activity include study of clinical significance of changes in the immune system in patients with CNS diseases (brain tumors, injury, neuro-infeciton, epilepsy, multiple encephalomyelitis, etc.), development of methods and approaches to immune correction of CNS diseases; study of anti-tumor immunity mechanisms; study of immunological properties of neural stem cells. Author of 76 scientific works, including 16 publications abroad, 2 Patents of Ukraine, 3 technical innovations, co-author of 4 monographs.

Senior researcher Liubych Larysa Dmytrievna -candidate of biological sciences. Key areas of research and practical activity – study of immunomodulating and anti-tumour properties of cells of embryonic and fetal brain, study of proapoptogenic and local immuno-suppression activity of CNS tumors, study of autoimmune reaction to brain antigens in patients with normal conditions and CNS pathologies. Author of 119 scientific works, including 32 publications abroad, 3 Patents of Ukraine, co-author of 5 monographs.

Senior researcher Belska Liudmyla Mykolaivna – candidate of biological sciences. Key areas of research and practical activity include study of systemic and local (in CNS tissue) changes in the state of phagocytes in patients with normal conditions, during neurotransplantation and with pathological conditions (gliomas, experimental allergic encephalomyelitis). Author of 74 scientific works, including 12 publications abroad, co-author of 2 monographs.

Research assistant Kliuchkova Antonina Ivanivna – key areas of research and practical activity include study of the dynamics of development of immune responses to intracerebral and intramuscular injection of alloantigens (experimental study). Author of 12 scientific works.

Publications

Monographs:

1. Ilichebych I.K. Lisianiy M.I., Yanchiy R.I. Antibodies and regulation of body functions. К. Naukova dumka.-1986.-246p.
2. Romodanov A.P., Lisianiy M.I. Traumatic brain injury and immunological reactivity of the body. К. Zdoroviye.-1985.-145p.
3. Romodanov А.P., Lisianiy M.I. and co-authors. Post-traditional encephalopathy. К. Zdoroviye.-1993.-221p.
4. Pedachenko E.H., Lisianiy M.I. and co-authors. Modern problems of pathogenesis of traumatic brain injury..К.- LLC “Zadruha”.-1996.-242p.
5. Zozulia Y.P., Lisianiy M.I. and co-authors. Chronic effect of small doses of irradiation on the nervous system. К. “Chornobyl inter form”.-1998.-480p.
6. Lisianiy N.I. Immune system of brain. К. Closed joint stock society “Vypol”.-1999.-213p.
7. Lisianiy M.I., Liubych L.D. Mechanisms of immunoneuronalopathological processes under the effect of radiation. К. Closed joint stock society “Vypol”.-2001.-198p..
8. Lisianiy M.I. Immunology and immune therapy of multiple sclerosis. К.- Closed joint stock society “Vypol”.—2003.-251p.
9. Zozulia Y.P., Lisianiy M.I. Neurogenetic differentiation of stem cells. К.- LLC “Eksab”.-2005.-363p.
10. Lisianiy M.I. Modern technologies of conservative treatment of gliomas. Brain gliomas (Under the editorship of Zozulia Y.A.) К.: “Ekspres-Polihraf” LLC.-2007.-383-569.

Articles:

11. Lisianiy M.I., Belska L.M., Lisianiy O.M, Fedorchyk O.H. The effect of in vitro amitosin and ВС-1 on CNS tumors of various genesis and immuno-competent cells // Immunology and allergology.-2007,#1.-p.42-46.
12. Lisianiy M.I., Belska L.M. Immuno-suppressive effect of malignant tumors // Ukrainian neurosurgery journal.-2007,#1.- p.4-9.
13. Semenova V.M., Liubych L.D., Lisianiy M.I., Petrenko A.Y., Stayno L.P. Proliferative and differential potential of neurocells of human embryo brain under culture conditions // Ukrainian neurosurgery journal.- 2007, ## 1.-p.68-71.
14. Zozulia Y.A., Semenova V.M., Cherchenko А.P., Liubych L.D., Stayno L.P., Drahuntseva N.H. Glial-enriched fraction of cultured neurocells as an object for intracerebral neurotransplantation // Academy of Medical Science Journal.-2007,#1.-V.13.-p.93-103.
15. V.I.Tsymbaliuk, L.І.Sokolova, М.І.Lisianiy, О.V.Markova, L.D.Pichkur, L.L.Chebotareva, V.А.Rudenko, О.Y.Chuvashova, L.D.Liubych, А.І.Tretianova, N.О.Haliarnyk, L.F.Syniavska, A.H.Potapova, І.А.Votiakova, S.О.Vasylovska, S.А.Verbovska. Motor functions and immune status of patients with multiple sclerosis after endolumbal introduction of allogenic fetal neuro-cells // Ukrainian neurology journal.-2007,# 1(2).-p.
16. Liubych L.D. Immunomodulating properties of embryonic brain cells (Literature review) // Immunology and allergology.-2007,# 3.-p.3-7.
17. Lisianiy M.I., Liubych L.D. Study of in vitro effect of supernatant of immature brain cells on the expression of cytokinesand antigens HLA with brain tumor cells and mononuclear cells of human peripheral blood // Transplantology.-2007,#1.-p.156-159.
18. Semenova V.M., Liubych L.D., Stayno L.P., Velychko O.P., Medvedev V.V. Intracerebral neurotransplantation of glia-enriched fraction of cultured neurocells – an object of study of remote post-transplantation effects // Transplantology.-2007,#1.-p.250-252.
19. Lisianiy M.I., Hnedkova I.A. Immunological properties of stem cells //|Neuroimmunology.–2007,#3-4.–p.4-12.
20. Prymushko L.І., SemenovaV.М., Lisianiy О.М., Tormasova А.І. Study of anti-tumor effect of immunomodulating drug galavit // Ukrainian neurosurgery journal.-2007,#1-.p.32-36.
21. Tsymbaliuk V.I., Markova О.V., Lisianiy M.I., Pichkur L.D., Sokolova L.І., Verbovska S.А., Chayka О.І. Treatment of different sex patients with multiple sclerosis by way of endolumbal introduction of halogen fetal neuro-cells// A collection of scientific works by workers of the Shupik National Medical Academy of Postgraduate Education.-К.-2007.-Issue.16, Book..1.- p.248-253.
22. Lisianiy M.I., Tormasova А.І. Specificity of immune response to alloantigen during intramuscular and intracerebral injection // Immunology and allergology.-2007,# 3.-p.46-49 // 2nd National congress on immunology, allergology abd immuno-rehabilitation “Modern achievements in clinical immunology and allergology”. November 19-23, Kyiv – Myrhorod.
23. Sokolova L.I., Lisianiy M.I., Kruhliak H.O. Study of herpes-viral persistence in patients with multiple sclerosis: specificity of clinical findings, diagnosis and treatment //Neyronauky: theoretical and clinical aspects.-V.3, #1-2.-1007.-P.80-84.

Diagnostic methods

1. Immune status:

1) homogenous antibodies panel (13 types)
2) functional activity of immune cells (with mitogens), killer activity, in vitro synthesis of cytokines, phagocytosis with latex, Nitro Blue Tetrazolium Reduction Test, myeloperoxidase activity, МТТ-test
3) identification of activation and apoptotic markers on immune, tumor and other cells
4) immunoglobulin content in blood, cryoglobulin content, immune complexes (general and differentiated).

2. Neuroimmune responses: level of antibodies vs. neuroantigens (ОБМ, S-100, acid glial proteid, neurospecific enolase), neuro sensibilization to the above-mentioned antigens.

3. Determination of levels of pro- and anti-inflammatory cytokines in blood, in supernatants (інтерлейкін-1; 4; 6; 8; 10; interferon-α, γ, ФНО- α , etc.).

4. ELISA for various viruses, immune antigens in blood and bodily fluids.

5. Polymerase chain reaction (DNA and RNA tests) to detect 20 types of agents causing neuroinfections, gnetic and malignant blood diseases.
Determination of expression of genes of apoptosis, interleukins, oncogenes, etc.

Types of pathology

1. Immunodeficiency and autoimmune diseases, including CNS diseases.

2. Neurooncological and neurovascular diseases (gliomas, medulloblastomas, ischemic and hemorrhagic lesions).

3. Epilepsies, hyperkinetic disorder, children cerebral palsy.

4. Multiple sclerosis, encephalopathy, polyneuropathy of different etiology;

5. Chronic fatigue syndrome, febrile states.

Counseling

The Neuroimmunolofy department staff provide counseling assistance. If you have any questions, you can emal to nimun.neuro@gmail.com

Contacts

Our address: 04050, Kyiv, 32 Platona Mayborody St., Institute of Neurosurgery, Neuroimmunology department (laboratory block, 5th floor). How to find us – take trolleybus #6, 18 from the “Lukyanivska” metro station to the stop “Bohoutivska Street”, then walk to Platona Mayborody street.

You can contact us by phone:
+38 044 483 81 93

e-mail:
nimun.neuro@gmail.com

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